Phospho-Caspase-9 (Tyr153) Rabbit Polyclonal Antibody

SKU: orb5766

Description

Phospho-Caspase-9 (Tyr153) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Caspase 9 around the phosphorylation site of Tyr153 LA(p-Y)IL
• Target: CASP9
• Molecular Weight: 35 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CASP9 | Caspase-9 (p-Y153); p-Caspase-9; phospho-Caspase-9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; CASP-9; Caspase-9; Casp-9-CTD; Casp9_v1; CASP9_HUMAN; CASP9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3 (APAF-3); ICE-like apoptotic protease 6 (ICE-LAP6); 3.4.22.62; Q4FJK5_MOUSE; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; Q920G4_RAT;

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice)). Primary Antibody: Rabbit Anti-Phospho-Caspase-9 (Tyr153)/FITC Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA. Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Caspase-9 (Tyr153)) Polyclonal Antibody, Unconjugated (orb5766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Caspase-9 (Tyr153) (orb5766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 35 kD.

Quick Database Links

Gene Symbol

CASP9

UniProt

P55211