Phospho-Caspase-9 (Ser196) Rabbit Polyclonal Antibody

• Catalog Number: orb4769
• Category: Antibodies
• Description: Phospho-Caspase-9 (Ser196) Rabbit Polyclonal Antibody
• Target: CASP9
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CASP9 around the phosphorylation site of Ser196 FS(p-S)LH
• UniProt ID: P55211
• RRID: AB_10922163
• MW: 35 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APAF 3; APAF3; Apoptosis related cysteine peptidas
• Note: For research use only

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Caspase-9 (Ser196)) polyclonal Antibody, Unconjugated (orb4769) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Caspase-9 (Ser196)) Polyclonal Antibody, Unconjugated (orb4769) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Rat) Lysate at 40 ug, Kidney (Rat) Lysate at 40 ug, Primary: Anti-Phospho-Caspase-9 (Ser196) (orb4769) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 35 kD, Observed band size: 35 kD.

Sample: Heart (Rat) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Caspase-9 (Ser196) (orb4769) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46-51/35/37 kD, Observed band size: 51/37 kD.