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Phospho-CaMKII (Thr287) Rabbit Polyclonal Antibody

Catalog Number: orb10223

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10223
CategoryAntibodies
DescriptionPhospho-CaMKII (Thr287) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityGallus, Mouse, Rabbit, Sheep
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Thr287 QE(p-T)VD
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW54 kDa
TargetCAMK2A
UniProt IDQ9UQM7
RRIDAB_10753118
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesphospho-CaMK2 alpha(Thr287); phospho-CaMK2 α(Thr28
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Background_CLIENT_OVERRIDE_
NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-CaMKII (Thr287) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (orb10223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMKII (Thr287) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (orb10223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMKII (Thr287) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (orb10223) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMKII (Thr287) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (orb10223) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.