Phospho C-Myc (Thr58 / Ser62) Rabbit Polyclonal Antibody

SKU: orb704368

Description

Phospho C-Myc (Thr58 / Ser62) Rabbit Polyclonal Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Myc around the phosphorylation site of Thr58 and Ser62 LP(p-T)PPL(p-S)PS
• Target: MYC
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MYC | c-Myc (p-T58/S62); p-c-Myc; phospho-c-Myc; MRTL; MYCC; bHLHe39; c-Myc; Myc2; Niard; Nird; RNCMYC; mMyc; MYC_HUMAN; MYC; Class E basic helix-loop-helix protein 39 (bHLHe39); Proto-oncogene c-Myc; Transcription factor p64; MYC_MOUSE; MYC_RAT;

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho C-Myc (Thr58 / Ser62)) Polyclonal Antibody, Unconjugated (orb704368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho C-Myc (Thr58 / Ser62)) Polyclonal Antibody, Unconjugated (orb704368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

MYC

UniProt

P01106