Phospho-c-Jun (Thr91) Rabbit Polyclonal Antibody

SKU: orb4918

Description

Phospho-c-Jun (Thr91) Rabbit Polyclonal Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Jun around the phosphorylation site of Thr91 TT(p-T)PT
• Target: JUN
• Molecular Weight: 43/36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

JUN | c-Jun (p-T91); p-c-Jun; phospho-c-Jun; AP-1; AP1; c-Jun; cJUN; p39; Junc; JUN_HUMAN; JUN; Activator protein 1 (AP1); Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; JUN_MOUSE; AH119; V-jun avian sarcoma virus 17 oncogene homolog (Jun A); JUN_PIG; JUN_RAT; Rjg-9;

Blank control (blue line): HepG2 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-c-Jun (Thr91) antibody (orb4918), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Thr91))) Polyclonal Antibody, Unconjugated (orb4918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

JUN

UniProt

P05412