Phospho-c-Fos (Thr325) Rabbit Polyclonal Antibody

• Catalog Number: orb5857
• Category: Antibodies
• Description: Phospho-c-Fos (Thr325) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325 LC(p-T)PV
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: FOS
• UniProt ID: P01100
• RRID: AB_10934634
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: c-Fos(phospho T325); c-Fos(phospho-Thr325); c-Fos(
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/AF488 Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P-c-Fos (Thr325)) Polyclonal Antibody, Unconjugated (orb5857) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: LOVO (Human) Cell Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Thr325) (orb5857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

Sample: MCF-7 (Human) cell Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Thr325) (orb5857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Thr325) (orb5857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-c-Fos (Thr325) Polyclonal Antibody, Unconjugated (orb5857) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.