Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody

• Catalog Number: orb156346
• Category: Antibodies
• Description: Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Ser362 KG(p-S)SS
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: FOS
• UniProt ID: P01100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: c-Fos(phospho S362); c-Fos(phospho-Ser362); c-Fos(
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Phospho-c-Fos (Ser362) Polyclonal Antibody, Unconjugated (orb156346) at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (orb156346) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (orb156346) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, A431 (Human) Cell Lysate at 30 ug, Adrenal glands (Mouse) Lysate at 40 ug, Adrenal glands (Rat) Lysate at 40 ug, Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Ser362) (orb156346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62/46 kD, Observed band size: 46 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Ser362) (orb156346) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.