Phospho-c-Fos (Ser32) Rabbit Polyclonal Antibody

• Catalog Number: orb5855
• Category: Antibodies
• Description: Phospho-c-Fos (Ser32) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Ser32 YH(p-S)PA
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: FOS
• UniProt ID: P01100
• RRID: AB_10921497
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: c-Fos(phospho S32); c-Fos(phospho Ser32); phospho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Ser32) /AF488 antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser32)) Polyclonal Antibody, Unconjugated (orb5855) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.