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Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody

Catalog Number: orb312170

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb312170
CategoryAntibodies
DescriptionPhospho-BRAF (Ser365) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit
ReactivityRat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human BRAF around the phosphorylation site of Ser365 SS(p-S)AP
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW84 kDa
TargetBRAF
UniProt IDP15056
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesB-Raf(phospho Ser365); B-Raf(phospho S365); B-Raf(
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (orb312170) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

  • Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody [orb5749]

    IF,  IHC-Fr,  IHC-P

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 200 μl, 100 μl
  • Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody (Cy5) [orb903269]

    IF

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Cy5

    100 μl
  • Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody (APC) [orb998871]

    IF

    Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit

    Rat

    Rabbit

    Polyclonal

    APC

    100 μl
  • Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody (Cy5.5) [orb1048868]

    IF

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Cy5.5

    100 μl
  • Phospho-BRAF (Ser365) Rabbit Polyclonal Antibody (RBITC) [orb1049078]

    IF

    Bovine, Canine, Equine, Gallus, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    RBITC

    100 μl