Phospho-Beta catenin (Tyr86) Rabbit Polyclonal Antibody

• Catalog Number: orb4778
• Category: Antibodies
• Description: Phospho-Beta catenin (Tyr86) Rabbit Polyclonal Antibody
• Target: CTNNB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Beta-catenin around the phosphorylation site of Tyr86 GQ(p-Y)AM
• UniProt ID: P35222
• RRID: AB_10922325
• MW: 86 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: beta Catenin(phospho Y86); beta catenin(phospho Ty
• Note: For research use only

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 0.3% tritionx-100 for 5 min at room temperature). Primary Antibody: Rabbit Anti-phospho-Beta catenin (Tyr86) antibody (orb4778, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were Primary antibody (orb4778, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20000 events was performed.

Blank control: 293T. Primary Antibody (green line): Rabbit Anti-phospho-Beta catenin (Tyr86) antibody (orb4778), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Beta catenin (Tyr86)) Polyclonal Antibody, Unconjugated (orb4778) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Beta catenin (Tyr86)) Polyclonal Antibody, Unconjugated (orb4778) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.