Phospho-Beta Catenin (Ser675) Rabbit Polyclonal Antibody

SKU: orb5771

Description

Phospho-Beta Catenin (Ser675) Rabbit Polyclonal Antibody

Research Area

Beta Catenin, Cadherins, Cancer Research, Cell Biology, Cytoplasmic, Immunology, Leukocyte Recruitment, Neurogenesis, Neuroscience

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Ser675 RL(p-S)VE
• Target: CTNNB1
• Molecular Weight: 86 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CTNB1_HUMAN; Catenin beta-1; catenin beta 1; CTNNB1; Catenin beta; Beta-catenin; CTNNB; OK/SW-cl.35; PRO2286; EVR7; MRD19; NEDSDV; armadillo; βcatenin; β catenin; β-catenin;

Blank control (blue line): HeLa (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Beta Catenin (Ser675) antibody (orb5771), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Beta Catenin (Ser675)) Polyclonal Antibody, Unconjugated (orb5771) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Beta Catenin (Ser675)) Polyclonal Antibody, Unconjugated (orb5771) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

Gene Symbol

CTNNB1

UniProt

P35222