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Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Catalog Number: orb506140

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb506140
CategoryAntibodies
DescriptionPhospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Gallus, Porcine, Rabbit, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthesised phosphopeptide derived from human beta Catenin around the phosphorylation site of Ser37 IH(p-S)GA
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW86 kDa
TargetCTNNB1
UniProt IDP35222
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesbeta Catenin(phospho S37); beta Catenin(phospho Se
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-beta Catenin (Ser37)) Polyclonal Antibody, Unconjugated (orb506140) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-beta Catenin (Ser37)) Polyclonal Antibody, Unconjugated (orb506140) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.