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Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Catalog Number: orb155826

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb155826
CategoryAntibodies
DescriptionPhospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody
TargetCTNNB1
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityGallus, Porcine, Rabbit
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthesised phosphopeptide derived from human beta Catenin around the phosphorylation site of Ser37 IH(p-S)GA
UniProt IDP35222
MW86 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesbeta Catenin(phospho S37); beta Catenin(phospho Se
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NoteFor research use only
Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Blank control: 293T. Primary Antibody (green line): Rabbit Anti-phospho-beta Catenin (Ser37) antibody (orb155826), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-beta Catenin (Ser37)) Polyclonal Antibody, Unconjugated (orb155826) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-beta Catenin (Ser37) Rabbit Polyclonal Antibody

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-phospho-beta Catenin (Ser37) (orb155826) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92 kD, Observed band size: 92 kD.