Phospho-Bcr (Tyr177) Rabbit Polyclonal Antibody (BF488)

• Catalog Number: orb2433056
• Category: Antibodies
• Description: Phospho-Bcr (Tyr177) Rabbit Polyclonal Antibody (BF488)
• Target: BCR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF488
• Predicted Reactivity: Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesisedphosphopeptide derived from human Bcr around the phosphorylation site of Tyr177 PF(p-Y)VN
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Bcr(phospho Y177); Bcr(phospho-Tyr177); ALL; BCR 1
• Research Area: Cancer Research, Cell Biology, Kinases, Ras Family
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-VNN1 antibody (orb2433056), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.