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Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Catalog Number: orb4671

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb4671
CategoryAntibodies
DescriptionPhospho-Bad (Ser134) Rabbit Polyclonal Antibody
TargetBAD
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser134 PK(p-S)AG
UniProt IDQ92934
RRIDAB_10937160
MW18 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesBad(phospho S134); Bad(phospho Ser134); p-Bad(S134
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Blank control (Black line): Jurkat (Black). Primary Antibody (green line): Rabbit Anti-Phospho-Bad (Ser134) antibody (orb4671), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Bad (Ser134) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Bad (Ser134)) Polyclonal Antibody, Unconjugated (orb4671) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.