Phospho-ATM (Tyr170) Rabbit Polyclonal Antibody

SKU: orb155758

Description

Phospho-ATM (Tyr170) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human ATM around the phosphorylation site of Tyr170 SV(p-Y)FR
• Target: ATM
• Molecular Weight: 370 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ATM(phospho-Tyr170); ATM(phospho Y170); p-ATM(Tyr170); p-ATM(Y170); A-T mutated; A-T mutated homolog; AT complementation group A; AT complementation group C; AT complementation group D; AT complementation group E; AT mutated; AT protein; AT1; ATA; Ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated gene; Ataxia telangiectasia mutated homolog(human); Ataxia telangiectasia mutated homolog; ATC; ATD; ATDC; ATE; ATM; ATM_HUMAN; DKFZp781A0353; Human phosphatidylinositol 3 kinase homolog; MGC74674; Serine protein kinase ATM; Serine-protein kinase ATM; Serine/threonine-protein kinase ATM; T cell prolymphocytic leukemia; Tefu; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; Telomere fusion protein; TPLL.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-ATM (Tyr170) antibody (orb155758), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

ATM

UniProt

Q13315