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Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody

Catalog Number: orb5735

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5735
CategoryAntibodies
DescriptionPhospho-ATG1 (Ser556) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, IHC-Fr, IHC-P
Predicted ReactivityEquine, Porcine
ReactivityHuman, Mouse, Rabbit, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human ATG1/ULK1 around the phosphorylation site of Ser556 LH(p-S)AP
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=3ug/test, ELISA=1:5000-10000
Form/AppearanceLiquid
ConjugationUnconjugated
MW116 kDa
TargetULK1
UniProt IDO75385
RRIDAB_10952506
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesULK1(phospho S556); ULK1(phospho Ser556); p-ULK1(S
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody

Blank control: A549. Primary Antibody (green line): Rabbit Anti-Phospho-ATG1 (Ser556) antibody (orb5735), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-ATG1 (Ser556)) polyclonal Antibody, Unconjugated (orb5735) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody

phosphopeptide, non phosphopeptide.

Phospho-ATG1 (Ser556) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-ATG1 (Ser556) Polyclonal Antibody, Unconjugated (orb5735) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.