Phospho-ATF2 (Thr69 / Thr51) Rabbit Polyclonal Antibody

• Catalog Number: orb155750
• Category: Antibodies
• Description: Phospho-ATF2 (Thr69 / Thr51) Rabbit Polyclonal Antibody
• Target: ATF2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr69 + Thr51 DQ(p-T)PT
• UniProt ID: P15336
• MW: 52 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: phospho-ATF2(Thr69)(human); phospho-ATF2(Thr51)(mo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Cerebellum (Mouse) Lysate at 40 ug, Uterus (Mouse) Lysate at 40 ug, Primary: Anti-phospho-ATF2 (Thr69/Thr51) (orb155750) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-ATF2 (Thr69 / Thr51) Polyclonal Antibody, Unconjugated (orb155750) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.