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Phospho-ATF2 (Ser62 / Ser44) Rabbit Polyclonal Antibody

Catalog Number: orb4590

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb4590
CategoryAntibodies
DescriptionPhospho-ATF2 (Ser62 / Ser44) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Human, Porcine, Rabbit
ReactivityMouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser44 / Ser62 (human) ND(p-S)VI
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW55 kDa
TargetATF2
UniProt IDP15336
RRIDAB_10996989
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesATF2(phospho S62+S44); ATF2(phospho-Ser44); ATF2(p
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-ATF2 (Ser62 / Ser44) Rabbit Polyclonal Antibody

Sample: Liver (Mouse) Lysate at 40 ug, Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-p-ATF2 (Ser44) (orb4590) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.

Phospho-ATF2 (Ser62 / Ser44) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ATF2 (Ser62 Ser44)) Polyclonal Antibody, Unconjugated (orb4590) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Phospho-ATF2 (Ser62 / Ser44) Rabbit Polyclonal Antibody

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-p-ATF2 (Ser44) (orb4590) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.