Phospho-Amyloid Precursor Protein (Ser730) Rabbit Polyclonal Antibody

• Catalog Number: orb4528
• Category: Antibodies
• Description: Phospho-Amyloid Precursor Protein (Ser730) Rabbit Polyclonal Antibody
• Target: APP
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human APP770 around the phosphorylation site of Ser730 YT(p-S)IH
• UniProt ID: P05067
• RRID: AB_10935889
• MW: 3.4-9/83 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Amyloid Precursor Protein(phospho S730); Amyloid P
• Research Area: DNA / RNA Binding, DNA and RNA Research, RNA Proce
• Note: For research use only

Blank control (blue line): Mouse Spleen cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 0.1% Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-phospho-Amyloid Precursor Protein (Ser730) antibody (orb4528), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (APP (Phospho-Ser730)) Polyclonal Antibody, Unconjugated (orb4528) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.