Phospho-AMPK alpha 1 (Ser496) Rabbit Polyclonal Antibody

• Catalog Number: orb506135
• Category: Antibodies
• Description: Phospho-AMPK alpha 1 (Ser496) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 1 around the phosphorylation site of Ser496 SG(p-S)VS
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 64 kDa
• Target: PRKAA1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: phospho-AMPK alpha-1(S496); AMPK alpha-1(phospho-S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha 1 (Ser496)) Polyclonal Antibody, Unconjugated (orb506135) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: MCF-7 (Human) Cell Lysate at 40 ug, PANC-1 (Human) Cell Lysate at 40 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser496) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.