Phospho-Alpha synuclein (Ser129) Rabbit Polyclonal Antibody

• Catalog Number: orb7038
• Category: Antibodies
• Description: Phospho-Alpha synuclein (Ser129) Rabbit Polyclonal Antibody
• Target: SNCA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SNCA around the phosphorylation site of Ser129 MP(p-S)EE
• UniProt ID: P37840
• RRID: AB_10952655
• MW: 15 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SNCA(phospho S129); alpha Synuclein(phospho S129);
• Note: For research use only

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-phospho-Alpha synuclein (Ser129) antibody (orb7038), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Cerebellum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 6: U251 (Human) Cell Lysate at 30 ug, Lane 7: 293T (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Alpha synuclein (Ser129) (orb7038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 18 kD.

Sample: Lane 1: Human SY5Y cell lysates, Lane 2: Human U87MG cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human HUVEC Cell Lysates, Lane 5: Human HELA Cell Lysates, Lane 6: Human 293T Cell Lysates, Primary: Anti-phospho-Alpha synuclein (Ser129) (orb7038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kDa, Observed band size: 18 kDa.

Sample: RAW264.7 (Mouse) Cell Lysate at 40 ug, Primary: Anti-phospho-Alpha synuclein (Ser129) (orb7038) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 15 kD.