Phospho-ALK (Tyr1604) Rabbit Polyclonal Antibody

• Catalog Number: orb5685
• Category: Antibodies
• Description: Phospho-ALK (Tyr1604) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Guinea pig, Porcine
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ALK around the phosphorylation site of Tyr1604 GH(p-Y)ED
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 174 kDa
• Target: ALK
• UniProt ID: Q9UM73
• RRID: AB_10936829
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ALK(phospho-Tyr1604); ALK(phospho-Y1604); p-ALK(Ty
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-phospho-ALK (Tyr1604) antibody (orb5685), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (ALK) Polyclonal Antibody, Unconjugated (orb5685) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Thyroid cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-ALK (Tyr1604) Polyclonal Antibody, Unconjugated (orb5685) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.