Phospho-14-3-3 protein zeta (Ser58) Rabbit Polyclonal Antibody

SKU: orb5643

Description

Phospho-14-3-3 protein zeta (Ser58) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 RS(p-S)WR
• Target: YWHAZ
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

phospho-14-3-3 protein zeta+delta(Ser58); 14-3-3 zeta(phospho S58); YWHAE(phospho S58); 14-3-3(phospho S58); YWHAZ(phospho S58); 14-3-3 zeta(phospho S58); p-14-3-3 zeta(phospho S58); 14 3 3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; 14-3-3 protein beta/alpha; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; YWHAB; YWHAZ; GW128; HS1; KCIP-1; YWHAE; YWHAA; 1433Z_HUMAN; Full=14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1.

Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 protein zeta + delta (Ser58) antibody (orb5643), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Hepg2 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-14-3-3 protein zeta (Ser58) (orb5643) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Quick Database Links

Gene Symbol

YWHAZ

UniProt

P31946