Cart summary

You have no items in your shopping cart.

Phospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit Polyclonal Antibody

Catalog Number: orb186708

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb186708
CategoryAntibodies
DescriptionPhospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit Polyclonal Antibody
TargetYWHAB
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184 LN(p-S)PE
UniProt IDP31946
MW28 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative names14-3-3 beta+zeta(phospho S184+S186); 14-3-3 beta+z
Read more...
NoteFor research use only
Phospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit Polyclonal Antibody

Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 beta + zeta (Ser186) antibody (orb186708), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Phospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit Polyclonal Antibody

Blank control: A431. Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 beta+zeta (Ser186/Ser184) antibody (orb186708), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YWHAB) Polyclonal Antibody, Unconjugated (orb186708) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (YWHAB) Polyclonal Antibody, Unconjugated (orb186708) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.