PHLDA1 Rabbit Polyclonal Antibody

SKU: orb11460

Description

PHLDA1 Rabbit Polyclonal Antibody

Research Area

Cell Cycle

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PHLDA1 (251-360/401aa)
• Target: PHLDA1
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Apoptosis associated nuclear protein; Apoptosis-associated nuclear protein; DT1P1B11; MGC131738; PHLA1_HUMAN; PHLDA1; PHRIP; Pleckstrin homology like domain family A member 1; Pleckstrin homology-like domain family A member 1; PQ rich protein; PQ-rich protein; PQR protein; Proline-and glutamine-rich protein; Proline-and histidine-rich protein; T cell death associated gene; T CELL DEATH ASSOCIATED GENE 51; T-cell death-associated gene 51 protein; Tdag; TDAG51.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PHLDA1) Polyclonal Antibody, Unconjugated (orb11460) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-PHLDA1 (orb11460) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 45 kDa.

Tissue/Cell: mouse pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PHLDA1/TDAG51 Polyclonal Antibody, Unconjugated (orb11460) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PHLDA1

UniProt

Q8WV24