PH20 Hyaluronidase Recombinant Protein, Tag Free

SKU: orb1671372

Description

PH20 Hyaluronidase Recombinant Protein, Tag Free

Images & Validation

• Tested Applications: WB
• Application Notes:
  This recombinant protein can be used for WB. For research use only.

Key Properties

• Source: HEK293 cells
• Biological Activity: The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 ˚C in a 2.0 mL reaction mixture) (QC tested).
• Target: SPAM1
• Tag: No Tag
• MW: 52.6 kDa
• Purity: >95% as determined by SDS-PAGE and >95% as determined by SEC-MALS.
• Protein Sequence: Leu 36 - Ser 490

Storage & Handling

• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5 with trehalose as protectant.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15

Quick Database Links

Gene Symbol

SPAM1

UniProt

P38567

RefSeq:AAH26163