PGK1 Rabbit Polyclonal Antibody

• Catalog Number: orb580297
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PGK1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Sheep, Yeast, Zebrafish
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PGK1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: PGK1
• UniProt ID: P00558
• Protein Sequence: Synthetic peptide located within the following region: PEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFR
• NCBI: NP_000282
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: PGKA, MIG10, HEL-S-68p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/ml of the antibody was used in this experiment. PGK1 is a highly abundant sample in most sample types with two or more possible isoforms at 45 kDa and 41 kDa. Use care in dilution considering the relative abundance of this protein in western blotting.

Sample Tissue: Mouse Kidney, Antibody dilution: 1 ug/ml.

Sample Type: Human Fetal Brain, Antibody dilution: 1.0 ug/ml.

WB Suggested Anti-PGK1 Antibody Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate. PGK1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.