PGC Antibody

• Catalog Number: orb1258055
• Category: Antibodies
• Description: PGC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Pepsinogen II (NP_001159896.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45-50kDa
• Target: PGC
• UniProt ID: P20142
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PEPC, PGII, gastricsin, pepsin C, pepsinogen C, pe
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Pepsinogen II antibody (orb1258055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.