Peroxiredoxin 3 Rabbit Polyclonal Antibody

• Catalog Number: orb13659
• Category: Antibodies
• Description: Peroxiredoxin 3 Rabbit Polyclonal Antibody
• Target: PRDX3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human PRDX3 (161-256/256aa)
• UniProt ID: P30048
• RRID: AB_10767954
• MW: 21 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Antioxidant protein 1; AOP 1; AOP-1; AOP1; HBC189;
• Research Area: Alzheimer's Disease, Cancer Research, Cardiovascul
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Peroxiredoxin 3) Polyclonal Antibody, Unconjugated (orb13659) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Peroxiredoxin 3) Polyclonal Antibody, Unconjugated (orb13659) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Liver tissue lysates, Lane 3: Mouse Lung tissue lysates, Lane 4: Rat Liver tissue lysates, Lane 5: Rat Lung tissue lysates, Lane 6: Rat Stomach tissue lysates, Lane 7: Human 293T cell lysates, Lane 8: Human A431 cell lysates, Lane 9: Human HepG2 cell lysates, Lane 10: Human HeLa cell lysates, Lane 11: Human A549 cell lysates, Primary: Anti-Peroxiredoxin 3 (orb13659) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 25 kDa.