Penicillinase Antibody Biotin Conjugated

SKU: orb344978

Description

Penicillinase antibody (Biotin)

Images & Validation

• Tested Applications: DOT
• Dilution range: IP: User Optimized, WB: User Optimized
• Reactivity: Bacteria
• Application Notes:
  Anti-Penicillinase Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Penicillinase [Enterobacter cloacae]
• Purity: Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae]. Cross reactivity against Penicillinase from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Penicillinase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase

Dot Blot results of Rabbit Anti-Penicillinase Antibody Biotin Conjugated. Dots are Penicillinase at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit Anti-Penicillinase Biotin at 1 µg/ml for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.

Quick Database Links

UniProt

P05364

RefSeq:AAO24600.1