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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 50000 cells/well are added to rhCD31 coated plates (3. 3 μg/mL, 100 μl/well) in the presence of 20 ng/mL PMA, approximately 55 - 65% will adhere after 30 minutes at 37˚C. |
| Target | PECAM1 |
| Tag | His Tag |
| MW | 66.4 kDa |
| Purity | >95% as determined by SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Gln 28 - Lys 601 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PECAM1, CD31, FLJ34100, FLJ58394
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
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