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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
| Target | PDHA1 |
| Molecular Weight | Observed: 45kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PDHA1, PDHA, PDHCE1A, PHE1A
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Western blot analysis of extracts of various cell lines, using PDHA1 antibody (orb1258402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using PDHA1 antibody (orb1258402) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using PDHA1 antibody (orb1258402) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PDHA1 antibody (orb1258402) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using PDHA1 antibody (orb1258402) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PDHA1 antibody (orb1258402) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
PDHA1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
PDHA1 Antibody (orb1258402)
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