PDGFRA Antibody

• Catalog Number: orb1263025
• Category: Antibodies
• Description: PDGFRA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Gallus, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 123 kDa
• Target: PDGFRA
• UniProt ID: P16234
• NCBI: P16234
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Platelet-derived growth factor receptor alpha, PDG
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35 ug/lane)

Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.