PDGFRA Antibody

• Catalog Number: orb1263024
• Category: Antibodies
• Description: PDGFRA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Gallus, Mouse, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from human PDGFRA.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 123 kDa
• Target: PDGFRA
• UniProt ID: P16234
• NCBI: P16234
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Platelet-derived growth factor receptor alpha, PDG
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Antibody staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:1000 dilution + human platelet lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of PDGFRA using rabbit polyclonal PDGFRA Antibody (Y720) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y720), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.