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Description
Research Area
Signal Transduction
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Biological activity was determined by the dose - dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0 - 2.0 ng/mL. |
| Target | PDGFB |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT |
Storage & Handling
−| Storage | The lyophilized PDGF-BB recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PDGF-BB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing PDGF-BB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
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Human PDGF-BB Protein [orb1471774]
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Greater than 98% as determined by reducing SDS-PAGE.
12.42 KDa
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10 μg, 50 μg Recombinant Mouse PDGF-BB Protein [orb3002372]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 13.4 KDa. Observed: 15 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human PDGF-BB Protein [orb3002491]
SDS-PAGE: Greater than 98% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 12.42 KDa. Observed: 14 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg- Item 1 of 1
- Item 1 of 1
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002599.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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