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Description
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Protein Sequence | SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT |
Storage & Handling
−| Storage | Stability: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | PDGF-BB was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human PDGF-BB Protein [orb1471774]
Unconjugated
Greater than 98% as determined by reducing SDS-PAGE.
12.42 KDa
Mammalian
10 μg, 50 μgRecombinant Human PDGF-BB Protein [orb3002491]
SDS-PAGE: Greater than 98% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 12.42 KDa. Observed: 14 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse PDGF-BB Protein [orb3002372]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 13.4 KDa. Observed: 15 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Protocol Information
PDGF BB Protein (orb429458)
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