PDGFB Antibody (Biotin)

SKU: orb1272339

Description

PDGFB Antibody (Biotin)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor-BB).
• Target: PDGFB
• Purification: Anti-hPDGF-BB specific antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B

To detect hPDGF-BB by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human PDGF-BB (orb1272340) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPDGF-BB.

To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

PDGFB

UniProt

P01127

RefSeq:P01127