PDGF-A Rabbit Polyclonal Antibody

• Catalog Number: orb11253
• Category: Antibodies
• Description: PDGF-A Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from the missle of human PDGF-A (125-170/211aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: PDGFA
• UniProt ID: P04085
• RRID: AB_10752475
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Platelet–dirived growth factor–1; PDGFA; PDGF AA;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): A431 cells (blue). Primary Antibody (green line): Rabbit Anti-PDGF-A antibody (orb11253), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: A549 (human) cell Lysate at 40 ug, Primary: Anti-PDGF-A (orb11253) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kD, Observed band size: 18 kD.

Sample: placenta (Mouse) Lysate at 40 ug, Primary: Anti-PDGF-A (orb11253) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kD, Observed band size: 17 kD.