PDE5A antibody

• Catalog Number: orb18971
• Category: Antibodies
• Description: Goat polyclonal antibody to PDE5A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Canine, Human, Rat
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 100; 94.8; 93.6
• Target: PDE5A
• Entrez: 8654
• Protein Sequence: LINGESGQAKRN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PDE5A antibody, anti CN5A antibody, anti PDE5
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 100kDa band observed in Human Lung lysates (calculated MW of 100kDa according to isoform 1, NP_001074.2). In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18971 staining (0.3ug/ml) of human lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing PDE5A (RC218974) and probed with orb18971 (mock transfection in first lane), tested by Origene.