PDE4D Rabbit Polyclonal Antibody

• Catalog Number: orb11252
• Category: Antibodies
• Description: PDE4D Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PDE4D (401-550/809aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 90 kDa
• Target: PDE4D
• UniProt ID: Q08499
• RRID: AB_10751702
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: cAMP specific 3', 5' cyclic phosphodiesterase 4D;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µgL 1X PBS containing 0.5% BSA (green).

Sample: Brain (Mouse) Lysate at 30 ug, Primary: Anti-PDE4D (orb11252) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 90 kD, Observed band size: 100 kD.

Sample: Brain (Mouse) Lysate at 30 ug, Primary: Anti-PDE4D (orb11252) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 90 kD, Observed band size: 90kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-PDE4D (orb11252) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90kD, Observed band size: 90 kD.