PDE1A Rabbit Polyclonal Antibody

SKU: orb312700

Description

PDE1A Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PDE1A (451-535/535aa)
• Target: PDE1A
• Molecular Weight: 61 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3' 5' cyclic nucleotide phosphodiesterase; 5''-cyclic nucleotide phosphodiesterase 1A; 61 kDa Cam PDE; 61 kDa Cam-PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A; Calcium/Calmodulin stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3''; Calmodulin-dependent phosphodiesterase; Cam PDE 1A; Cam-PDE 1A; CAM-PDE-1A; HCAM 1; hCam-1; HCAM1; HSPDE1A; MGC26303; PDE 1A; PDE1A; PDE1A_HUMAN; phosphodiesterase 1A; phosphodiesterase 1A calmodulin dependent.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDE1A) Polyclonal Antibody, Unconjugated (orb312700) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDE1A) Polyclonal Antibody, Unconjugated (orb312700) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-PDE1A (orb312700) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 61 kD.

Quick Database Links

Gene Symbol

PDE1A

UniProt

P54750