PDCD6IP Rabbit Polyclonal Antibody

SKU: orb101359

Description

PDCD6IP Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PDCD6IP (601-700/868aa)
• Target: PDCD6IP
• Molecular Weight: 95 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PDC6I_HUMAN; Programmed cell death 6-interacting protein; AIP1; ALIX; KIAA1375; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95;

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-ALIX (orb101359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95-110 kD, Observed band size: 100 kD.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PDCD6IP

UniProt

Q8WUM4