PDCD6 Antibody

SKU: orb1256849

Description

PDCD6 Antibody

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1).
• Target: PDCD6
• Molecular Weight: Observed: 16kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed cell death protein 6, Apoptosis-linked gene 2 protein, Probable calcium-binding protein ALG-2, PDCD6, ALG2

Western blot analysis of extracts of various cell lines, using PDCD6 antibody (orb1256849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using PDCD6 antibody (orb1256849) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using PDCD6 antibody (orb1256849). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug PDCD6 antibody (orb1256849). Western blot was performed from the immunoprecipitate using PDCD6 antibody (orb1256849) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

PDCD6

UniProt

O75340