PDCD4 antibody

• Catalog Number: orb12312
• Category: Antibodies
• Description: Goat polyclonal to PDCD4.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 51.7; 50.6; 50.2
• Target: PDCD4 (aa210-221)
• Entrez: 27250
• Protein Sequence: EGKASHREMTSK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PDCD4 antibody
• Note: For research use only
• Application notes: WB: Approx 50kDa band observed in lysates of cell line HeLa (calculated MW of 50.2kDa according to NP_001186421.1). Recommended concentration: 0.1-0.3µg/ml.. IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.
• Expiration Date: 12 months from date of receipt.

orb12312 (0.1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb12312 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.