PDCD4 Antibody

• Catalog Number: orb402438
• Category: Antibodies
• Description: PDCD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human PDCD4 recombinant protein (Position: E346-L446). Human PDCD4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat PDCD4, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51735 MW
• UniProt ID: Q53EL6
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Programmed cell death protein 4;Neoplastic transfo
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-PDCD4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of PDCD4 using anti-PDCD4 antibody.Lane 1:human Daudi cell; 2:human HeLa cell; 3:human 293T cell.

IHC analysis of PDCD4 using anti-PDCD4 antibody. PDCD4 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue.

IHC analysis of PDCD4 using anti-PDCD4 antibody. PDCD4 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinomaa tissue.

IHC analysis of PDCD4 using anti-PDCD4 antibody. PDCD4 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue.