PDCD1LG2 Rabbit Polyclonal Antibody

SKU: orb13269

Description

PDCD1LG2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC
• Dilution range: ICC/IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PDCD1LG2 (41-150/273aa)
• Target: PDCD1LG2
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PD L2; PD-L2; PD1L2_HUMAN; B7 dendritic cell molecule; B7-DC; B7DC; bA574F11.2; Btdc; Butyrophilin B7 DC; Butyrophilin B7-DC; Butyrophilin B7DC; CD 273; CD273; CD273 antigen; MGC142238; MGC142240; PD 1 ligand 2; PD-1 ligand 2; PD1 ligand 2; PDCD 1 ligand 2; PDCD1 ligand 2; PDCD1L2; PDL 2; PDL2; Programmed cell death 1 ligand 2; Programmed death ligand 2.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PDCD1LG2 antibody (orb13269), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (orb13269) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

PDCD1LG2

UniProt

Q9BQ51