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Catalog Number | orb2642797 |
---|---|
Category | Antibodies |
Description | PD-L2/PDCD1LG2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CDLA273-1 |
Tested applications | FACS, IF, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This PD-L2 antibody is available for research use only. |
UniProt ID | Q9BQ51 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the PD-L2 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of 1) human Raji and 2) mouse RAW264.7 cell lysate with PD-L2 antibody (clone CDLA273-1). Expected molecular weight: 31-60 kDa depending on glycosylation level.
Flow cytometry testing of human Jurkat cells with PD-L2 antibody (clone CDLA273-1); Red=isotype control, Blue= PD-L2 antibody.
Immunofluorescent staining of human Jurkat cells with PD-L2 antibody (green, clone CDLA273-1) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
ELISA, FACS, IF, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |