PD-L1 Antibody / B7-H1 / CD274

• Catalog Number: orb750313
• Category: Antibodies
• Description: Mouse monoclonal antibody against PD-L1 conjugated to Biotin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Biotin
• Reactivity: Human
• Buffer/Preservatives: 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A portion of amino acids 39-191 from the human protein was used as the immunogen for the PD-L1 antibody.
• UniProt ID: Q9NZQ7
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml at RT
• Application notes: Optimal dilution of the PD-L1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PDL1/2746
• Formula: 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the PD-L1 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term).
• Hazard Information: This PD-L1 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE lung carcinoma with biotin-conjugated PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE lung carcinoma with biotin-conjugated PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using PD-L1 antibody (clone PDL1/2746). These results demonstrate the foremost specificity of the PDL1/2746 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2746) as confirmation of integrity and purity.