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PD-L1 Antibody / B7-H1 / CD274

Catalog Number: orb639693

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb639693
CategoryAntibodies
DescriptionPD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
Species/HostMouse
ClonalityMonoclonal
Clone NumberPDL1/2743
Tested applicationsELISA, FACS, IF
ReactivityHuman
IsotypeMouse IgG2b, kappa
ImmunogenA portion of amino acids 39-191 from the human protein was used as the immunogen for this PD-L1 antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis PD-L1 antibody is available for research use only.
UniProt IDQ9NZQ7
StorageStore the PD-L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
PD-L1 Antibody / B7-H1 / CD274

Flow cytometry testing of human Jurkat cells with PD-L1 antibody (clone PDL1/2743); Red=isotype control, Blue= cells alone, Orange= PD-L1 antibody.

PD-L1 Antibody / B7-H1 / CD274

Immunofluorescent staining of human Jurkat cells with PD-L1 antibody (clone PDL1/2743, green) and Reddot nuclear stain (red).

PD-L1 Antibody / B7-H1 / CD274

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PD-L1 antibody (clone PDL1/2743). These results demonstrate the foremost specificity of the PDL1/2743 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

PD-L1 Antibody / B7-H1 / CD274

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2743) as confirmation of integrity and purity.

  • PD-L1 Antibody / B7-H1 / CD274 [orb606675]

    ELISA,  FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • PD-L1 Antibody / B7-H1 / CD274 [orb2641417]

    ELISA,  FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • PD-L1 Antibody / B7-H1 / CD274 [orb699708]

    IHC-P,  WB

    Human

    Mouse

    Recombinant

    Unconjugated

    20 μg
  • PD-L1 Antibody / B7-H1 / CD274 [orb2644677]

    IHC-P,  WB

    Human

    Mouse

    Recombinant

    Unconjugated

    100 μg
  • PD-L1 Antibody / B7-H1 / CD274 [orb750313]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Biotin

    500 μl